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The antibody is from purified rabbit serum by affinity purification by using sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

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Overexpression of this gene is observed in several tumors, which ends up in chromosome instability, and therefore might add to tumorigenesis. This protein was observed to associate with, and become involved with, the phosphorylation of NPAT protein (nuclear protein mapped for the ATM locus), which participates in mobile-cycle controlled histone gene expression and performs a important position in marketing mobile-cycle development from the absence of pRB. Two alternatively spliced transcript variants of this gene, which encode distinctive isoforms, are described. Two supplemental splice variants have been reported but specific nucleotide sequence details is not still accessible.

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Blocking peptides are peptides that bind particularly to your target antibody and block antibody binding. These peptide typically has the epitope recognized by the antibody.

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This guideline outlines even further attainable optimization of cellular and immunoassay parameters to ensure the very best final results are...

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This manual outlines more achievable optimization of cellular and immunoassay parameters to make certain the absolute best final results are...

Antibodies certain to the blocking peptide not bind for the epitope around the focus on protein. By comparing the staining from your blocked antibody vs . the antibody on your own, one can see which staining is unique.

Concentrate on Facts Cyclin E belongs on the hugely conserved cyclin loved ones, whose associates are characterized by a extraordinary periodicity in protein abundance from the cell cycle. Cyclins function as regulators of CDK kinases. Distinctive cyclins show distinct expression and degradation designs which add on the temporal coordination of each mitotic party. Cyclin E varieties a complex with and capabilities as a regulatory subunit of CDK2, whose action is required for cell cycle G1/S changeover. This protein accumulates at the G1-S period boundary which is degraded as cells progress by way of S section.

The measurement of protein phosphorylation is a great tool for measuring the modulation of receptor activation by the two antibodies...

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